Product description

DfCell Mycoplasma RT-qPCR Detection Kit is a rapid qualitative test based on Nucleic Acid Amplification Techniques (NAT) designed to detect potential Mycoplasma contamination in raw materials, cell banks, virus seeds, virus or cell harvests, and therapeutic cell products. This assay kit utilizes quantitative PCR technology employing a multiplex PCR approach with two fluorescent probes, FAM and VIC, to respectively detect the target sequence and internal reference. It covers over 100 Mycoplasma DNA sequences and has undergone rigorous validation for specificity, detection limit, and durability according to EP 2.6.7 standards. The detection limit meets the requirement of ≤10 CFU/mL, demonstrating high sensitivity, excellent specificity, and safety.

 

Specifications

Product Number	C230106E / C230106S
Specifications	25 T / 100 T

 

Components

Component Number	Component Name	C230106E	C230106S
C230106-A	4×qPCR Reaction Buffer	250 μL	1 mL
C230106-B	Primer & Probe Mix	25 μL	100 μL
C230106-C	Internal Control (IC)	25 μL	100 μL
C230106-D*	Positive Control (PC)	500 μL	2 mL
C230106-E**	DNA Dilution Buffer	1 mL	4×1 mL
C230106-F***	ddH2O	500 μL	2×1 mL

*PC：Positive control solution, with a concentration of 1, 000 copies/µL;

**DNA Dilution Buffer: Used for sample and IC dilution, as well as template for NTC and NC;

***ddH₂O: Used for preparing qPCR Mix system.

 

Storage

Store at temperatures ranging from -15°C to -25°C with a shelf life of one year.

Please note that component C230106-B should be stored protected from light.