Product description

Human BMP-4 ELISA (Enzyme-Linked Immunosorbent Assay) Kit is an in vitro enzyme-linked immunosorbent assay kit used for quantitative determination of BMP-4 in serum, plasma, and cell culture supernatants. Specific anti-BMP-4 antibodies are pre-coated onto high-affinity enzyme plates. Standards and samples are added to the wells of the enzyme plate, and after incubation, BMP-4 present in the sample binds to the solid-phase antibody. After washing to remove unbound substances, a detection antibody is added for binding and incubated, followed by washing, and then the enzyme complex (Streptavidin-HRP) is added for binding and incubated. After washing, a colorimetric substrateTMB is added for color development, avoiding light. The intensity of the color reaction is proportional to the concentration of BMP-4 in the sample. The reaction is terminated by adding a stop solution, and the absorbance is measured at 450 nm wavelength (with a reference wavelength of 570 - 630 nm).

Bone Morphogenetic Protein 4(BMP4) belongs to the TGF-β superfamily, playing essential roles in many developmental processes, including neurogenesis, vascular development, angiogenesis and osteogenesis. BMP-4 induces cartilage and bone formation. Also acts in mesoderm induction, tooth development, limb formation and fracture repair. Acts in concert with PTHLH/PTHRP to stimulate ductal outgrowth during embryonic mammary development and to inhibit hair follicle induction.

 

Specifications

Item Number	P162015S / P162015E
Specification	48 T / 96 T
Detection Range	15.63-1000 pg/mL
Detection Method	Sandwich ELISA
Detection Time	4.5 hours
Sensitivity	11.2 pg/mL
Dilution Linearity	86 - 127%
Recovery Rate	75 - 126%
Intra-assay Variability	4.0%
Inter-assay Variability	5.0%

 

Components

Component Number	Component Name	Storage Temperature	P162015E	P162015S
P162015-A	Plate	2~8℃	48 T	96 T
P162015-B	Standard	2~8℃	1 tube	2 tubes
P162015-C	Detection Antibody	2~8℃	60 μL	120 μL
P162015-D	Enzyme Conjugate	2~8℃(Avoid Light)	30 μL	60 μL
P162015-E	Sample Dilution Buffer	2~8℃	8 mL	15 mL
P162015-F	Antibody/Enzyme Dilution Buffer	2~8℃	15 mL	30 mL
P162015-G	20x Wash Buffer	2~8℃	25 mL	50 mL
P162015-H	Substrate Solution	2~8℃(Avoid Light)	8 mL	15 mL
P162015-I	Stop Solution	Room Temperature	5 mL	10 mL
P162015-J	Plate Sealers	Room Temperature	3 pieces	5 pieces

 

Storage

The assay kit can be stored at 2~8℃. Alternatively, the reagents can be stored according to the storage conditions provided in the component information to avoid contamination and repeated freeze-thaw cycles. Diluted working solutions should be used immediately and not reused. The shelf life is 1 year.

Table 1. Reagent Storage Table After First Use

Material Name	Storage Conditions
Plate	Unused strips can be returned to the aluminum foil bag, tightly sealed, and stored at 2~8°C to avoid moisture absorption.
Standard	Use within 48 hours after dissolution, store at 2~8°C to avoid contamination.
Detection antibody	Use within 48 hours after dilution, store at 2~8°C to avoid contamination.
Enzyme conjugate
Sample Dilution Buffer	Store at 2~8°C for 1 month, avoiding contamination.
20x Wash Buffer
Antibody/Enzyme Dilution Buffer
Substrate solution	Store at 2~8°C for 1 month, avoiding light exposure.
Stop solution	Can be stored at room temperature.
Plate Sealers