Product description

Mouse IL-12p70 ELISA (Enzyme-Linked Immunosorbent Assay) Kit is an in vitro enzyme-linked immunosorbent assay kit used for quantitative determination of IL-12p70 in serum, plasma, and cell culture supernatants. Specific anti-IL-12p70 antibodies are pre-coated onto high-affinity enzyme plates. Standards and samples are added to the wells of the enzyme plate, and after incubation, IL-12p70 present in the sample binds to the solid-phase antibody. After washing to remove unbound substances, a detection antibody is added for binding and incubated, followed by washing, and then the enzyme complex (Streptavidin-HRP) is added for binding and incubated. After washing, a colorimetric substrateTMB is added for color development, avoiding light. The intensity of the color reaction is proportional to the concentration of IL-12p70 in the sample. The reaction is terminated by adding a stop solution, and the absorbance is measured at 450 nm wavelength (with a reference wavelength of 570 - 630 nm).

Interleukin-12 (IL-12) is a heterodimeric pro-inflammatory cytokine best known for inducing IFN-γ production in T cells and NK cells. IL-12 consists of two disulfide-linked subunits (p35 and p40) that regulate the biosynthesis of IL-12p70. The effects of IL-12 are mediated through its high-affinity receptor that contains two subunits: IL-12Rβ1 and IL-12Rβ2. IL-12 is produced by macrophages and B cells and has been shown to have multiple effects on T cells and natural killer (NK) cells. While mouse IL-12 is active on both human and mouse cells, human IL-12 is not active on mouse cells.

 

Specifications

Item Number	P162016S / P162016E
Specification	48 T / 96 T
Detection Range	31.25-2000 pg/mL
Detection Method	Sandwich ELISA
Detection Species	Mouse
Detection Time	4.5 hours
Sensitivity	7.19 pg/mL
Dilution Linearity	96 - 124%
Recovery Rate	82 - 122%
Intra-assay Variability	3.2%

 

Components

Component Number	Component Name	Storage Temperature	P162016S	P162016E
P162016-A	Plate	2~8℃	48 T	96 T
P162016-B	Standard	2~8℃	1 tube	2 tubes
P162016-C	Detection Antibody	2~8℃	120 μL	240 μL
P162016-D	Enzyme Conjugate	2~8℃(Avoid Light)	30 μL	60 μL
P162016-E	5× Dilution Buffer	2~8℃	8 mL	15 mL
P162016-F	20× Wash Buffer	2~8℃	25 mL	50 mL
P162016-G	Substrate Solution	2~8℃(Avoid Light)	8 mL	15 mL
P162016-H	Stop Solution	Room Temperature	5 mL	10 mL
P162016-I	Plate Sealers	Room Temperature	3 pieces	5 pieces

 

Storage

The assay kit can be stored at 2~8℃. Alternatively, the reagents can be stored according to the storage conditions provided in the component information to avoid contamination and repeated freeze-thaw cycles. Diluted working solutions should be used immediately and not reused. The shelf life is 1 year.

Table 1. Reagent Storage Table After First Use

Material Name	Storage Conditions
Plate	Unused strips can be returned to the aluminum foil bag, tightly sealed, and stored at 2~8°C to avoid moisture absorption.
Standard	Use within 48 hours after dissolution, store at 2~8°C to avoid contamination.
Detection antibody	Use within 48 hours after dilution, store at 2~8°C to avoid contamination.
Enzyme conjugate
5×Dilution Buffer	Store at 2~8°C for 1 month, avoiding contamination.
20×Wash Buffer
Substrate solution	Store at 2~8°C for 1 month, avoiding light exposure.
Stop solution	Can be stored at room temperature.
Plate Sealers