Product description

The Annexin V-FITC/PI Apoptosis Detection Kit uses FITC-labeled Annexin V as a probe to detect the onset of early apoptosis. The detection principle is as follows: in normal living cells, phosphatidylserine (PS) is located on the inner side of the cell membrane, but in early apoptotic cells, PS flips from the inner side of the cell membrane to the surface of the cell membrane and is exposed to the extracellular environment. Annexin V, a calcium-dependent phospholipid-binding protein with a molecular weight of 35-36 kDa, has high affinity for PS and can bind to the membrane of early apoptotic cells through the exposed PS on the outer side of the cell membrane.In addition, Propidium Iodide (PI) is provided in this kit to distinguish surviving early cells from necrotic or late apoptotic cells.PI is a nucleic acid dye that does not penetrate through the intact cell membranes of normal or early apoptotic cells, but can penetrate through the cell membranes of late apoptotic and necrotic cells and stain the nuclei red. Therefore, when Annexin V was used in combination with PI, PI was excluded from living cells (Annexin V-/PI-) and early apoptotic cells (Annexin V+/PI-), while late apoptotic and necrotic cells stained double-positively (Annexin V+/PI+) with both FITC and PI.

This kit can be used for detection with flow cytometry or fluorescence microscopy.

 

Specifications

Components No.	C331406E	C331406S	C331406M
Size	20 T	50 T	100 T

 

Shipping and Storage

Transportation of ice packs: Store at -20°C in the dark to avoid repeated freezing and thawing, effective for one year.

[Note]If multiple reuses are required within a short period, store in the dark at 4°C, effective for six months.