Product description

Human IL-8 ELISA Kit is an in vitro enzyme-linked immunosorbent assay kit used for the quantitative determination of Human IL-8 (Interleukin-8) in serum and cell culture supernatants. Specific antibodies against Human IL-8 are pre-coated on high-affinity enzyme plates. Standard samples and test samples are added to the wells of the enzyme plate and, after incubation, the IL-8 present in the samples binds to the solid-phase antibody. After washing to remove unbound substances, a detection antibody is added and incubated to bind. Following another wash, an enzyme conjugate (Streptavidin-HRP) is added and incubated to bind. After washing, aTMB chromogenic substrate is added for color development in the absence of light. The intensity of the color reaction is proportional to the concentration of IL-8 in the sample. The reaction is terminated by adding a stop solution, and the absorbance is measured at 450 nm wavelength (with a reference wavelength of 570 - 630 nm).

IL-8, also known as CXCL8, is produced by macrophages and epithelial cells. It is a member of the α or CXC chemokine family and has a molecular weight of 8-9 kDa. IL-8 is a chemotactic factor that can attract neutrophils, eosinophils, and T cells, but not monocytes. It also plays a role in neutrophil activation and is released from several cell types in response to inflammatory stimuli.

 

Specifications

Item Number	P162022S / P162022E
Specification	48 T / 96 T
Detection Range	7.81-500 pg/mL
Detection Method	Sandwich ELISA
Detection Species	Human
Detection Time	5 hours
Sensitivity	0.382 pg/mL
Dilution Linearity	83.1 - 109.8%
Recovery Rate	73.7 - 112.4%
Intra-assay Variability	8.4%
Inter-assay Variability	7.0%

 

Components

Component Number	Component Name	Storage Temperature	P162022S	P162022E
P162022-A	Plate	2~8℃	48 T	96 T
P162022-B	Standard	2~8℃	1 tube	2 tubes
P162022-C	Detection Antibody	2~8℃	60 μL	120 μL
P162022-D	Enzyme Conjugate	2~8℃(Avoid Light)	30 μL	60 μL
P162022-E	5x Dilution Buffer	2~8℃	12 mL	25 mL
P162022-F	20x Wash Buffer	2~8℃	25 mL	50 mL
P162022-G	Substrate Solution	2~8℃(Avoid Light)	8 mL	15 mL
P162022-H	Stop Solution	Room Temperature	5 mL	10 mL
P162022-I	Plate Sealers	Room Temperature	3 pieces	5 pieces

 

Storage

The assay kit can be stored at 2~8℃. Alternatively, the reagents can be stored according to the storage conditions provided in the component information to avoid contamination and repeated freeze-thaw cycles. Diluted working solutions should be used immediately and not reused. The shelf life is 1 year.

Table 1. Reagent Storage Table After Initial Use

Material Name	Storage Conditions
Plate	Unused strips can be returned to the aluminum foil bag, tightly sealed, and stored at 2~8°C to avoid moisture absorption.
Standard	Use within 48 hours after dissolution, store at 2~8°C to avoid contamination.
Detection antibody	Use within 48 hours after dilution, store at 2~8°C to avoid contamination.
Enzyme conjugate
5x Dilution Buffer	Store at 2~8°C for 1 month, avoiding contamination.
20x Wash Buffer
Substrate solution	Store at 2~8°C for 1 month, avoiding light exposure.
Stop solution	Can be stored at room temperature.
Plate sealing film