Product description

Human MMP-9 ELISA Kit is an in vitro enzyme-linked immunosorbent assay kit used for the quantitative determination of Human Matrix Metalloproteinase-9 (MMP-9) in serum, plasma, and cell culture supernatants. Specific antibodies against Human MMP-9 are pre-coated on high-affinity enzyme plates. Standard samples and test samples are added to the wells of the enzyme plate and, after incubation, the MMP-9 present in the samples binds to the solid-phase antibody. After washing to remove unbound substances, a detection antibody is added and incubated to bind. Following another wash, an enzyme conjugate (Streptavidin-HRP) is added and incubated to bind. After washing, a TMB chromogenic substrate is added for color development in the absence of light. The intensity of the color reaction is proportional to the concentration of MMP-9 in the sample. The reaction is terminated by adding a stop solution, and the absorbance is measured at 450 nm wavelength (with a reference wavelength of 570 - 630 nm).

MMP-9, also known as Gelatinase B, 92 kDa type IV collagenase, 92 kDa gelatinase, or type V collagenase, is a glycosylated proenzyme. MMP-9 degrades components of the extracellular matrix (ECM) with high activity against denatured collagen (gelatin). It cleaves natural collagens of types III, IV, V, and XI, as well as elastin, nidogen-1, and vitronectin. MMP-9 can also cleave various chemokines and growth factors (such as IL-1β, CXCL 8/IL-8, CXCL 7, CXCL 4, CXCL 1, latent transforming growth factor-β, membrane-bound tumor necrosis factor-α, vascular endothelial growth factor, and basic fibroblast growth factor), amyloid precursor protein, substance P, and myelin basic protein. This activity can either increase or decrease the biological activity of soluble factors and can also release them from their association with the ECM.

 

Specifications

Item Number	P162025S / P162025E
Specification	48 T / 96 T
Detection Range	31.25-2000 pg/mL
Detection Method	Sandwich ELISA
Detection Species	Human
Detection Time	5 hours
Sensitivity	7.44 pg/mL
Dilution Linearity	76.7 -105.9 %
Recovery Rate	76.1 - 119.2 %
Intra-assay Variability	4.6 %
Inter-assay Variability	5.1 %

 

Components

Component Number	Component Name	Storage Temperature	P162025S	P162025E
P162025-A	Plate	2~8℃	48 T	96 T
P162025-B	Standard	2~8℃	1 tube	2 tubes
P162025-C	Detection Antibody	2~8℃	1 tube	2 tubes
P162025-D	Enzyme Conjugate	2~8℃（Avoid Light)	170 μL	350 μL
P162025-E	5×Dilution Buffer	2~8℃	12 mL	25 mL
P162025-F	20×Wash Buffer	2~8℃	25 mL	50 mL
P162025-G	Substrate Solution	2~8℃（Avoid Light)	8 mL	15 mL
P162025-H	Stop Solution	Room Temperature	5 mL	10 mL
P162025-I	Plate Sealers	Room Temperature	3 pieces	5 pieces

 

Storage

The assay kit can be stored at 2~8℃. Alternatively, the reagents can be stored according to the storage conditions provided in the component information to avoid contamination and repeated freeze-thaw cycles. Diluted working solutions should be used immediately and not reused. The shelf life is 1 year.

Table 1. Reagent Storage Table After Initial Use

Material Name	Storage Conditions
Plate	Unused strips can be returned to the aluminum foil bag, tightly sealed, and stored at 2~8°C to avoid moisture absorption.
Standard	Use within 48 hours after dissolution, store at 2~8°C to avoid contamination.
Detection antibody
Enzyme conjugate	Use within 48 hours after dilution, store at 2~8°C to avoid contamination.
5×Dilution Buffer	Store at 2~8°C for 1 month, avoiding contamination.
20×Wash Buffer
Substrate solution	Store at 2~8°C for 1 month, avoiding light exposure.
Stop solution	Can be stored at room temperature.
Plate sealing film