Product description

Percoll is a versatile, flexible and easy-to-use low-density gradient solution for the separation of cells, subcellular particles, bacteria, viruses, and even damaged cells and their fragments from intact living cells. It mainly consists of silica particles coated with a monolayer of polyvinylpyrrolidone (PVP), with a diameter of 15~30 nm, and the content of free PVP accounts for only 1~2%. Due to the heterogeneity of the pellet size, the centrifugation process precipitates at different rates, spontaneously generating a homogenized and isotonic gradient with densities ranging from 1.0 and 1.3 g/mL. Most biological particles with sedimentation coefficients >60S can be separated with Percoll.

 

It is a sterile solution, colorless to light yellow in color, with the following characteristics:

1. Percoll forms gradient densities ranging from 1.0~1.3 g/mL, and the entire gradient system is isotonic;

2. Non-cytotoxic, chemically inert, does not adhere to cell membranes;

3.Adjustable to physiological ionic strength and pH;

4.Cells, subcellular particles, and larger viruses (down to ~70S) can be separated under mild conditions, preserving their viability and morphological integrity.

5.Pre-formed or spontaneously formed gradients can be separated by centrifugation at medium speed with only an angular rotor head;

6.Percoll's low viscosity (10±5 cP at 20°C) enables rapid gradient formation and particle separation; satisfactory separation results can be achieved in only minutes to tens of minutes using preformed gradients separated by low-speed centrifugation (200~1000×g);

7.Percoll is highly stable, unopened products are valid for 5 years at room temperature; opened products are valid for at least 2 years of aseptic storage at room temperature;

8.Percoll (undiluted) can be re-autoclaved at high pressure, 120°C for 30 minutes.

 

Components

Components No.	C230136E
Size	100 mL

 

Shipping and Storage

Transport at room temperature. Store unopened at 4~30°C, with a shelf life of 5 years. After opening, store under aseptic conditions at 2~8°C.